Here we developed a panel of surface markers and an analysis strategy that accurately identify all known populations of macrophages and DCs, and their precursors in the lung during steady-state conditions and bleomycin-induced injury… … Dendritic cells (DCs), which serve both as innate lung sentinels and as orchestrators of adaptive immunity, are well equipped to drive both pathological processes from the earliest stages of COPD pathogenesis to end-stage disease. Human peripheral blood mononuclear cell (PBMC)-derived dendritic cells stained with Brilliant Violet 510™ anti-human CD11c antibody (clone 3.9, yellow). Dendritic cells (DCs) are a functionally, developmentally and phenotypically diverse group of cells. However, a systematic flow cytometric approach to identify subsets of macrophages and dendritic cells (DCs) accurately and consistently in the normal mouse lung has not been described. Dendritic cells (DCs), which serve both as innate lung sentinels and as orchestrators of adaptive immunity, are well equipped to drive both pathological processes from the earliest stages of COPD pathogenesis to end-stage disease. DC form a rare cell population in the lung and early studies were hampered because scarce cell populations are seldom easy to isolate. This clone is effective for use in IHC and FC. Lung dendritic cells were identified by immunohistochemistry in lung tissue sections from C57BL/6 mice. In addition to being potent stimulators/activators of an adaptive immune response DCs can also initiate an innate immune response and even have a role in immune tolerance (Schraml & Sousa 2015). CD11c, in combination with other markers (e.g. The dendritic cells (DC) are the most potent antigen-presenting cell. Lung DCs colocalized with CD4 + T cells, and expression of DC activation markers correlated with expression of CD69, an early and transient marker of T-cell activation, on CD4 + T cells from the same patients. Lung tumors likely employ numerous strategies to suppress DC-based anti-tumor immunity. Nuclei were counterstained with DAPI (blue). Dendritic cells (DCs) are the key factors providing protective immunity against lung tumors and clinical trials have proven that DC function is reduced in lung cancer patients. CD141), is used for visualization of human dendritic cells. Following isolation from the lungs using CD11c magnetic beads, the flow cytometric analysis of I-Ab+and CD11c+cells indicated a mixed population of dendritic cells at different stages of maturation, with most expressing an immature phenotype. It is evident that the immunoregulatory network may play a key role in the failure of the immune response to terminate tumors. Besides, this cell is pheno-typically heterogeneous depending on its localization, differentiation, or activation status. Cells of the innate immune system, and especially myeloid cells such as neutrophils, eosinophils, monocytes, macrophages (alveolar and interstitial), and dendritic cells (DCs, i.e., plasmacytoid DCs, CD103+DCs, and CD11b+DCs), play an important role in lung development and physiology, and contribute to important lung diseases, including pulmonary infection, cancer, asthma, chronic obstructive …
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